Coding

Part:BBa_K2619104:Design

Designed by: shiyu liu   Group: iGEM18_XJTLU-CHINA   (2018-09-08)


Nanoluciferase with C/D box sequence and 4*VEEV RNA stabilizing sequence at the 3' UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 253
    Illegal XhoI site found at 611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 69
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no specific considerations


Source

Nano luciferase:INOUYE, S., SATO, J.-I., SAHARA-MIURA, Y., YOSHIDA, S., KURAKATA, H. & HOSOYA, T. 2013. C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: The mutated catalytic 19kDa component of Oplophorus luciferase. Biochemical and Biophysical Research Communications, 437, 23-28. C/D box: Haloferax volcanii VEEV: Venezuelan equine encephalitis virus


References